Journal: Annals of Translational Medicine
Article Title: Liposomal honokiol inhibits glioblastoma growth through regulating macrophage polarization
doi: 10.21037/atm-21-1836
Figure Lengend Snippet: liposomal honokiol (Lip-HNK) regulates M1/M2 polarization via the STAT1/6 pathway. Interferon γ (IFN-γ) (20 ng/mL) and lipopolysaccharide (LPS) (10 ng/mL) with Lip-HNK were used to treat RAW264.7 (A) and BV2 (B) cells for 48 hours. p-STAT1, activators of transcription (STAT1), and signal transducers p(Y701)-STAT1, p(S727)-STAT1, STAT1 were evaluated by Western blot. RAW264.7 (C) and BV2 (D) cells were treated with interleukin 4 (IL-4) (20 ng/mL) and indicated concentrations of Lip-HNK. p-STAT6 and STAT6 were evaluated using Western blot analysis. The 3 independent experiments are illustrated by histogram bars. The data are displayed as the mean ± standard deviation. *, P<0.05; **, P<0.01; ***, P<0.001. #, P<0.05; ##, P<0.01.
Article Snippet: Antibodies against the following proteins were used: phosphor-STAT1 Tyr701 , phosphor-STAT1 ser727 , STAT1, STAT6, phosphor-STAT6 Tyr641 , and GAPDH (Cat. #8826, #9167, #14994, #56554, #5397, and #5174, respectively, Cell Signaling Technology, USA).
Techniques: Western Blot, Standard Deviation